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Cano, Irene; Ryder, David; Webb, Steve C.; Jones, Brian J.; Brosnahan, Cara L.; Carrasco, Noelia; Bodinier, Barbara; Furones, Dolors; Pretto, Tobia; Carella, Francesca; Chollet, Bruno; Arzul, Isabelle; Cheslett, Deborah; Collins, Evelyn; Lohrmann, Karin B.; Valdivia, Ana L.; Ward, Georgia; Carballal, María J.; Villalba, Antonio; Marigómez, Ionan; Mortensen, Stein; Christison, Kevin; Kevin, Wakeman C.; Bustos, Eduardo; Christie, Lyndsay; Green, Matthew; Feist, Stephen W.. |
Intracellular microcolonies of bacteria (IMC), in some cases developing large extracellular cysts (bacterial aggregates), infecting primarily gill and digestive gland, have been historically reported in a wide diversity of economically important mollusk species worldwide, sometimes associated with severe lesions and mass mortality events. As an effort to characterize those organisms, traditionally named as Rickettsia or Chlamydia-like organisms, 1950 specimens comprising 22 mollusk species were collected over 10 countries and after histology examination, a selection of 99 samples involving 20 species were subjected to 16S rRNA gene amplicon sequencing. Phylogenetic analysis showed Endozoicomonadaceae sequences in all the mollusk species analyzed.... |
Tipo: Text |
Palavras-chave: Mollusk; OTU; Endozoicomonas; Chlamydiae; Mycoplasma; Francisella; Lucinoma; Ridgeia. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00658/77032/78322.pdf |
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Lasa, Aide; Cesare, Andrea; Tassistro, Giovanni; Borello, Alessio; Gualdi, Stefano; Furones, Dolors; Carrasco, Noelia; Cheslett, Deborah; Brechon, Amanda; Paillard, Christine; Bidault, Adeline; Pernet, Fabrice; Canesi, Laura; Edomi, Paolo; Pallavicini, Alberto; Pruzzo, Carla; Vezzulli, Luigi. |
Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 (OsHV‐1) have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens microbial infections in C. gigas may derive from the contribution of a larger number of microorganisms than previously thought, according to an emerging view supporting the polymicrobial nature of bivalve disease. In this study, the microbial communities associated with a large number of C. gigas samples collected during recurrent mortality episodes at different European sites were investigated by real‐time PCR and 16SrRNA gene‐based microbial profiling. A new target enrichment next‐generation sequencing protocol for selective... |
Tipo: Text |
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Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00508/61952/66036.pdf |
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Carrasco, Noelia; Lopez, Immaculada; Alcaraz, M; Furones, M. Dolores; Berthe, Franck; Arzul, Isabelle. |
The life cycle of the species forming the genus Marteilia (Phylum Paramyxea), which are marine protozoan parasites of molluscs, is still poorly known, although there is evidence of the need for intermediate hosts. To date, only the calanoid copepod Acartia (Paracartia) grani has been demonstrated to be involved in the life cycle of the parasite infecting flat oysters growing in ponds with a low biodiversity. However, no complex natural environments, such as bays or estuaries, have been studied for a similar purpose. Therefore, a survey for the presence of the protozoan Marteilia was conducted by PCR on the zooplankton community of a natural ecosystem, the Alfacs and Fangar bays in the Delta de l'Ebre (NW Mediterranean). Identification of zooplankton... |
Tipo: Text |
Palavras-chave: Zooplankton; Harpaticoida sp.; Oithona sp.; Mollusc parasite; Life cycle; Marteilia. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3050.pdf |
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Escobedo-fregoso, C.; Arzul, Isabelle; Carrasco, Noelia; Gutierrez-rivera, J. N.; Llera-herrera, Raul; Vazquez-juarez, R.. |
Prevalence of the protozoan Perkinsus spp. in the gills of the pleasure oyster Crassostrea corteziensis from two estuaries in Nayarit, Mexico, was measured. The protozoan was identified by PCR amplification of the internal transcribed spacer (ITS) region of the rDNA of Perkinsus spp. The pathogen was found in 92% of oysters from Boca de Camichín and 77% of oysters from Pozo Chino. ITS sequences characterized from C. corteziensis showed 96–100% similarity to Perkinsus marinus. The most frequent ITS sequence, (GenBank JQ266236) had 100% identity with the ITS locus of P. marinus from New Jersey, Maryland, South Carolina, and Texas, and the second most frequent observed sequence (GenBank JQ266240) was 100% identical to ITS sequences of P. marinus from New... |
Tipo: Text |
Palavras-chave: Crassostrea corteziensis; Perkinsus marinus; Internal transcribed spacer; Non-internal transcribed spacer; Aquatic disease. |
Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00137/24811/22892.pdf |
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